Journal of Zhejiang University SCIENCE
(ISSN 1009-3095, Monthly)
2002   Vol. 3   No. 5   p.606-610

Preparation of protein samples for gel electrophoresis by sequential extraction

ZHONG Bo-xiong(ÖÓ²®ÐÛ)(College of Animal Sciences, Zhejiang University, Hangzhou 310029, China)  
WENG Hong-biao(Î̺êì®)(College of Animal Sciences, Zhejiang University, Hangzhou 310029, China)  
FANG Wei-huan(·½Î¬»À)(College of Animal Sciences, Zhejiang University, Hangzhou 310029, China)  

Abstract:Since preparation and solubilization of protein samples are crucial factors in proteome research, the authors established a sequential extraction technique to prepare protein samples from the body wall of the 5th instar larvae of silkworm, Bombyx mori. Two kinds of protein samples were obtained from the body wall using the method. Between the two types of samples only about 15% proteins were identical; the majority were different, indicating that more species of proteins could be obtained with the sequential extraction method; which will be useful for preparation of protein samples for proteome study.
Keywords:Proteome, Sequential extraction, 2D-electrophoresis, Protein spot match, Amino acid sequence

CLC Number:Q503  Document ID:A

Author Resume:ZHONG Bo-xiong(ÖÓ²®ÐÛ),E-mail: bzhong@mail.hz.zj.cn

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Manuscript Received:2001 June 20

Manuscript Revised:2002 April 18

Published:2002 Dec. 1