Journal of Zhejiang University SCIENCE
(ISSN 1009-3095, Monthly)
2002   Vol. 3   No. 5   p.617-621

Rapid detection of chromosome 18 aneuploidies in amniocytes by using primed in situ labeling (PRINS) technique

YANG Jian-bin(杨建滨)(Cancer Institute, Second Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310009, China)  
ZHENG Shu(郑树)(Cancer Institute, Second Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310009, China)  

Abstract:This paper presents a feasible method for rapid detection of the interphase nuclei of uncultured amniocytes for chromosomes 18 by using our modified primed in situ labeling (PRINS) technique. A total of 262 independent, uncultured amniotic fluid samples were analysed in a blind fashion before the karyotype was available. In addition, 62 samples were examined by fluorescence in situ hybridization (FISH) for comparison. In more than 95% of the samples PRINS reactions with primer 18cen were successfully induced. Two samples were properly identified and correctly scored as trisomic 18. PRINS reaction could be performed automatically in less than one hour with a programmable thermocycler. Our studies showed that the PRINS technique is simple, rapid and cost-effective. It is as sensitive and specific as FISH; can enhance the accuracy of standard cytogenetic analysis; and allows identification of chromosomes 18 aneuploidies in uncultured amniocytes in significantly less time.
Keywords:Primed in situ labeling (PRINS), Prenatal diagnosis, Chromosome 18

CLC Number:Q987;R394.2  Document ID:A

Foundation Item:Project supported by the Scientific Research Foundation for Returned Overseas Chinese Scholars, State Education Ministry and Postdoctoral Research Foundation of China
Author Resume:YANG Jian-bin(杨建滨),E-mail: bqyjb@mail.hz.zj.cn; ZHENG Shu(郑树),zhengshu@mail.hz.zj.cn

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Manuscript Received:2002 June 12

Manuscript Revised:2002 Aug. 23

Published:2002 Dec. 1