Journal of Zhejiang University SCIENCE
(ISSN 1009-3095, Monthly)
2003   Vol. 4   No. 3   p.369-373

Polymorphisms in the genes for coagulation factor II,V,VII in patients undergoing coronary angiography

XU Geng(徐耕)(The Second Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310009, China)
JIN Guo-dong(金国栋)(The Second Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310009, China)
FU Guo-sheng(傅国胜)(The Second Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310009, China)
MA Ji(马骥)(The Second Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310009, China)
SHAN Jiang(单江)(The Second Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310009, China)
WANG Jia-nan(王建安)(Sir Run Run Shaw Hospital, College of Medicine, Medicine, Zhejiang University, Hangzhou 310009, China)

Abstract:Objective: To determine whether polymorphisms in the genes for coagulation factor II,V, VII could predispose an individual to increase risk for coronary artery disease (CAD) and/or myocardial infarction (MI) in Chinese. Methods: We screened coagulation factor II(G20210A),V(G1691A),VII (R353Q and HVR4) genotype in 374 patients undergoing coronary angiography by polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) assay. Results: The R353Q and HVR4 genotype of the factor VII distribution was in accordance with Hardy-Weinberg equilibrium. The frequencies of FVII genotype or allele did not show statistically significant differences between CAD group and controls or between male and female. The frequencies of the Q allele and (RQ+QQ) genotype were significantly higher among the CAD patients without myocardial infarction (MI) history than among those with MI history (P<0.05). However, HVR4 polymorphism was not significantly different within groups. We only find one normal control of factorII(G20210A) mutation. No coagulation factor V(G1691A) mutation was found in the CAD patients and controls. Conclusion: The factor II(G20210A),V(G1691A) mutation is absent and may not be a major genetic factor for CAD and/or MI; the Q allele of the R353Q polymorphism of the factor VII gene may be a protective genetic factor against myocardial infarction in Chinese.
Keywords:Coagulation factor, Polymorphism, Coronary angiography, Myocardial infarction

CLC Number:R541.4 Document ID:A

Foundation Item:Project(No.021103166) supported by a grant from the Key Project of Science and Technology Commission of Zhejiang Province, China
Author Resume:XU Geng,E-mail: xugengzju@21cn.com

References:

[1]Bertina, R.M., Koeleman, P.C., Koster, T., Rosendaal, F.R.,Dirven, R.J., De Ronde, H., Velden, P.A. and Reitsma, P.H., 1994. Mutation in blood coagulation factor V associated with resistance to activated protein C. Nature, 369:64-67.
[2]Feraresi, P., Machetti, C., Legnani, C., Cavallari, E., Castoldi, E., Mascoli, F., Ardissino, D., Palareti, E. and Bernardi, F., 1997. The heterozygous 20210 G/A prothrombin genotype is associated with early venous in inherited thrombophilias and is not increased in frequency in artery disease. Arterioscler Thromb Vasc Biol, 17:2418-2422.
[3]Girelli, D., Russo, C. and Ferraresi, P., 2000. Polymorphisms in the factor VII gene and the risk of myocardial infarction in patients with coronary artery disease. N Engl J Med, 343: 774-780.
[4]Green, F.G., Kellecher, C., Wilkes, H., Temple, A., Meade, D. and Humphries, S., 1991. A common genetic polymorphism associated with lower coagulation factor VII levels in healthy individuals. Arterioscler Thromb, 11 : 540-546.
[5]Marchetti, G., Gemmati, D. and Patracchini, P., 1991. PCR detection of a repeat polymorphism within the F7 gene. Nucleic Acids Res, 19 : 4570.
[6]Poort, S., Rosendaal, F.R., Reitsma, P.H. and Bertina, R., 1996. A common genetic variation in the 3'-untranslated region of the prothrombin levels and an increase in venous thrombosis. Blood, 88: 3698-3703.
[7]Redondo, H.H., Watzke, B., Stucki, I., Sulzer, F., Demarmels, Biasiutti, B.R., Binder, M., Furlan, B. and Lammle, W.A., 1999. Coagulation factor II,V,VII, andⅩ, prothrombin gene 20210G→A transition, and factor V Leiden in coronary artery disease. Arterioscler Thromb Vasc Biol, 19:1020-1025.
[8]Ridker, P.M., Hennekens, C.H., Lindpaintner, K., Stampfer, M.J., Eisenberg, P.R. and Miletich, J.P., 1995. Mutation in the gene coding for coagulation factor V and the risk of myocardial infarction, stroke, and venous thrombosis in apparently healthy men. N Engl J Med, 332: 912-917.
[9]Ridker, P.M., Hennekens, C.H. and Miletich, J.P. 1999. G20210A mutation in prothrombin gene and risk of myocardial infarction, stroke, and venous thrombosis in a large cohort of US men. Circulation, 99: 999-1004.
[10]Rosendaal, F.R., Siscovick, D.S., Schwartz, S.M., Psaty, M.Y., Raghunathan, T.E. and Vos, H.L., 1997. A common prothrombin variant (20210 G to A) increase the risk of myocardial infarction in young women. Blood, 90: 1747-1750.
[11]Song, J., Yoon, Y.M. and Jung, H.J., 2000. Plasminogen activator inhibitor-1 4G/5G promoter polymorphism in Kroean patients with coronary artery disease. J. Kroean Med Sci., 15 (2) :146-152.
[12]Watzke, H.H., Schuttrumpf, J., Graf, S., Huber, K. and Panzer, S., 1997. Increased prevalence of a polymorphism in the gene coding for human prothrombin in patients with coronary heart disease. Thromb Res, 87: 521-526.
[13]Wu, A.H. And Tsongalis, G.J., 2001. Correlation of polymorphisms to coagulation and biochemical risk factors for cardiovascular disease. Am J. Cardiol, 87:1361-1366

Manuscript Received:2002年8月28日

Manuscript Revised:2002年12月10日

Published:2003年6月1日