Journal of Zhejiang University SCIENCE
(ISSN 1009-3095, Monthly)
2003   Vol. 4   No. 4   p.491-501

Correlation of matrix metalloproteinase-2, -9, tissue inhibitor-1 of matrix metalloproteinase and CD44 variant 6 in head and neck cancer metastasis

XU Ya-ping(徐娅苹)(The Second Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310009, China)
ZHAO Xue-qun(赵学群)(The Second Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310009, China)
SOMMER, K.(Lübeck Medical University, Lübeck 23538, Germany)
MOUBAYED, P.(Lübeck Medical University, Lübeck 23538, Germany)

Abstract:This study aimed to explore the molecular mechanism in tumor invasion and metastasis. The expression of matrix metalloproteinase-2,-9 (MMP-2, MMP-9), tissue inhibitor-1 of matrix metalloproteinase (TIMP-1), cell adhesion molecule 44 variant 6 (CD44v6), HER2/neu and p53 was investigated in 154 patients with head and neck squamous cell carcinoma (SCC) by ABC and ImmunoMax immunohistochemical method. Their clinical relevance and correlation were analysed. The expression of MMP-2, MMP-9, TIMP-1, CD44v6, HER2/neu and p53 was found in cancer cells in 87.01%, 85.71%, 68.18%, 98.05%, 55.19% and 50.65% cases respectively. Linear regression and correlation analysis revealed that there was close positive relationship (P<0.05) between the expression of MMP-2 and MMP-9, TIMP-1 and CD44v6, HER2/neu and MMP-9, MMP-2 and p53. Up-regulation of MMP-2 was accompanied by advanced T stage(P<0.01). There was also a trend of MMP-2 expression being related with tumor metastasis. Increased expression of HER2/neu was found in patients with tumor recurrence(P<0.05). The expression of TIMP-1 was higher in laryngeal cancer than that in pharyngeal cancer, and higher in keratinizing and non-keratinizing SCC than that in basaloid SCC(P<0.05). These findings suggested that MMP-2 and MMP-9, HER2/neu and MMP-9, MMP-2 and p53 had a coordinate function in aggression of tumor; that MMP-2 had a more important function than MMP-9 in tumor invasion and metastasis; and that HER2/neu might serve as a biomarker for poor prognosis in HNSCC.
Keywords:Head and neck cancer, Matrix metalloproteinase-2, -9 (MMP-2 and MMP-9), Tissue inhibitor-1 of matrix metalloproteinase (TIMP-1), Cell adhesion molecule 44 variant 6 (CD44 v6), HER2/neu, p53

CLC Number:R76 Document ID:A

Foundation Item:Project supported by Lubeck Medical University ,Germany
Author Resume:XU Ya-ping (徐娅苹),E-mail: xuyap@yahoo.com

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Manuscript Received:2002 Aug. 30

Manuscript Revised:2002 Dec. 12

Published:2003 Aug. 30