Journal of Zhejiang University SCIENCE
(ISSN 1009-3095, Monthly)

2004   Vol. 5   No. 12   p.1613-1620

Mathematical modeling of salt-gradient ion-exchange simulated moving bed chromatography for protein separations

LU Jian-gang

(National Laboratory of Industrial Control Technology, Institute of Industrial Process Control, Zhejiang University, Hangzhou 310027, China)
E-mail: JGLU@iipc.zju.edu.cn
Received Oct. 8, 2003; revision accepted Feb. 28, 2004

Abstract: The salt-gradient operation mode used in ion-exchange simulated moving bed chromatography (SMBC) can improve the efficiency of protein separations. A detailed model that takes into account any kind of adsorption/ion-exchange equilibrium, salt gradient, size exclusion, mass transfer resistance, and port periodic switching mechanism, was developed to simulate the complex dynamics. The model predictions were verified by the experimental data on upward and downward gradients for protein separations reported in the literature. All design and operating parameters (number, configuration, length and diameter of columns, particle size, switching period, flow rates of feed, raffinate, desorbent and extract, protein concentrations in feed, different salt concentrations in desorbent and feed) can be chosen correctly by numerical simulation. This model can facilitate the design, operation, optimization, control and scale-up of salt-gradient ion-exchange SMBC for protein separations.

Key words: Simulated moving bed chromatography, Salt gradients, Size exclusion, Proteins, Mathematical model
doi:10.1631/jzus.2004.1613             CLC number: O657.7; TQ460.35