Journal of Zhejiang University SCIENCE
(ISSN 1009-3095, Monthly)

2005   Vol. 6B   No. 2   p.132-136

Representative appressorium stage cDNA library of Magnaporthe grisea

LU Jian-ping, LIU Tong-bao, YU Xiao-yun, LIN Fu-cheng^*

(School of Life Sciences, Zhejiang University, Hangzhou 310029, China)
^*E-mail: fuchenglin@zju.edu.cn
Received Apr. 29, 2004; revision accepted June 15, 2004

Abstract: A mature appressorium cDNA library of rice blast fungus, Magnaporthe grisea, was constructed in a λTriplEx2 vector by SMART^TM cDNA library containing 2.37×10⁶ independent clones about 100% of which harbor foreign cDNA inserts with average size of 660 bp. Of 9 randomly selected clones, 2 expressed sequence tags (ESTs) sequences did not have homologous EST sequences of M. grisea in GenBank. The appressorium cDNA library is suitable for gene expression analysis and function analysis of the late stages of appressorium formation and the early stages of penetration of M. grisea.

Key words: Magnaporthe grisea, Appressorium, cDNA library
doi:10.1631/jzus.2005.B0132             CLC number: Q949.32