Journal of Zhejiang University SCIENCE
(ISSN 1009-3095, Monthly)

2005   Vol. 6B   No. 7   p.622-625

Effects of fructose-1,6-diphosphate on concentration of calcium and activities of sarcoplosnic Ca²⁺-ATPase in cardiomyocytes of Adriamycin-treated rats

CAI Wei^†, CHEN Jun-zhu, RUAN Li-ming, WANG Yi-na

(Department of Internal Medicine, First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310003, China)
^†E-mail: weic236@sohu.com
Received Oct. 18, 2004; revision accepted Dec. 25, 2004

Abstract: Objective: To observe the effects of fructose-1,6-diphosphate (FDP) on serum levels of cardiac troponin I (cTnI) and creatine kinase-MB (CK-MB), as well as the concentration of calcium in cardiomyocytes (Myo[Ca²⁺]) and activity of sarcoplosnic Ca²⁺-ATPase (SRCa²⁺-ATPase) in Adriamycin (ADR)-treated rats. Methods: Rats were intraperitoneally injected with ADR (2.5 mg/kg every other day for 6 times) and then with different dosages of FDP (every other day for twenty-one times). Bi-antibodies sandwich Enzyme linked immune absorption assay (ELISA) was performed to detect serum level of cTnI. CK-MB was detected by monoclonal antibody, Myo[Ca²⁺] was detected by fluorescent spectrophotometry and the activity of SRCa²⁺-ATPase was detected by inorganic phosphate method. Results: FDP (300, 600, 1200 mg/kg) significantly reduced the serum levels of cTnI and CK-MB, while at the same time decreased calcium concentration and increased SRCa²⁺-ATPase activity in cardiomyocytes of ADR-treated rats (P<0.01). Conclusions: FDP might alleviate the cardiotoxic effects induced by ADR through decreasing calcium level as well as increasing SRCa²⁺-ATPase activity in cardiomyocytes.

Key words: Fructose-1,6-diphosphate, Adriamycin, Cardiomyocyte, Calcium, Sarcoplosnic reticulum Ca²⁺-ATPase
doi:10.1631/jzus.2005.B0622             CLC number: R331