Journal of Zhejiang University SCIENCE
(ISSN 1009-3095, Monthly)
2005 Vol. 6B No. 10 p.1015-1021
Investigation on the origin of 5-HMF in Shengmaiyin decoction by RP-HPLC method
LI Ying-hua, LU Xiu-yang†‡
(Institute of Pharmaceutical Engineering, Zhejiang University, Hangzhou 310027, China)
†E-mail: luxiuyang@zju.edu.cn
Received May 17, 2005; revision accepted July 1, 2005
Abstract: The origin of 5-HMF (5-hydroxymethyl-2-furaldehyde) in a Shengmaiyin decoction was investigated by the RP-HPLC method below. A C18 column (250 mm×4.6 mm, i.d. 5 μm) with a column temperature of 25 °C was used. The mobile phase was a mixture of ultra-pure water-acetonitrile (95:5, V/V) and the flow rate was 1.0 ml/min. The detection wavelength was 280 nm. The injection volume was 1 μl and the running time was about 20 min. The addition of Schisandra was regulated to assess the contribution of an acid environment to the production of 5-HMF. In order to confirm the role of saccharides in the production of 5-HMF, different amount of fructose was used. The 5-HMF level in decoctions of processed and unprocessed Schisandra was investigated in order to determine the origin of 5-HMF. The results showed that 5-HMF was derived mainly from the decoction of Schisandra only and not the mixed decoction of Ophionpogon and Schisandra. The appearance of 5-HMF is not simply the result of the decomposition of saccharides under the acid environment created by Schisandra, but the processing procedure plays an important role in the production of 5-HMF.
Key words: Shengmaiyin, 5-HMF, Origin, Schisandra, Process
doi:10.1631/jzus.2005.B1015 CLC number: R289.1; TQ041+.7
‡ Corresponding Author