Journal of Zhejiang University SCIENCE B
(ISSN 1673-1581, Monthly)

2005   Vol. 6   No. 12   p. 1148-1152

Determination of theophylline concentration in serum by chemiluminescent immunoassay

Zhou Mei-xia^†1, Guan Cha-ying¹, Chen Guang¹, Xie Xin-you¹, Wu Sheng-hai²

(¹Department of Laboratory, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou 310016, China)
(²Department of Laboratory, Hangzhou First Hospital, Hangzhou 310006, China)
^†E-mail: zmx00018@126.com
Received July 25, 2005 revision accepted Nov. 3, 2005

Abstract: Objective: This study aimed to establish chemiluminescent immunoassay (CLIA) for quantitative determination of theophylline levels in human serum. Methods: To measure the concentration of theophylline (n=122) and evaluate the assay. Results: The linear range of the CLIA method was 0.51~40 mg/L (Y=1.02X+0.44, r=0.995). The intra and inter CV (coefficient variance) of CLIA were 3.20% and 3.57%, respectively. The average recovery rate was 102.3%. This method was free from interference by brilirubin (<200 μmol/L), hemoglobin (<10 g/L), and triglycerides (<15 mmol/L). Conclusion: This method is simple, convenient and precise for clinical pharmacokinetics study of theophylline.

Key words: Theophylline, Chemiluminescent immunoassay (CLIA), Fluorescence polarization immunoassay (FPIA)
doi:10.1631/jzus.2005.B1148             CLC number: R331.1

References:

[1] Amal, L., Bergmann, P., 2004. Evaluation of a chemiluminescence immunoassay for the determination of intact parathyroid hormone using the ADVIA Centaur. Clin. Lab., 50(11-12):695-702.

[2] Dasgupta, A., Datta, P., 2004. Rapid detection of oleander poisoning using digoxin immunoassays: comparison of five assays. Ther. Drug Monit., 26(6):658-663.

[3] Dawson, A.H., Whyte, I.M., 1999. Therapeutic drug monitoring in drug overdose. Br. J. Clin. Pharmacol., 48(3):278-283.

[4] Feng, Z., Li, Z., Sui, B., Xu, G., Xia, T., 2005. Serological diagnosis of infectious mononucleosis by chemiluminescent immunoassay using capsid antigen p18 of Epstein-Barr virus. Clin. Chim. Acta, 354(1-2):77-82.

[5] Gupta, S.V., McGowen, R.M., Callewaert, D.M., Brown, T.R., Li, Y., Sarkar, F.H., 2005. Quantitative chemiluminescent immunoassay for NF-kappaB-DNA binding activity. J. Immunoassay Immunochem., 26(2):125-143.

[6] Li, J., Mo, H., Huang, H., 2000. Effect of low dose of inhaled corticosteroid combined with small dose of oral theophylline on treatment of bronchial asthma. Zhonghua Jie He He Hu Xi Za Zhi, 23(6):336-339 (in Chinese).

[7] Lin, S., Han, S.Q., Liu, Y.B., Xu, W.G., Guan, G.Y., 2005. Chemiluminescence immunoassay for chloramphenicol. Anal. Bioanal. Chem., 382(5):1250-1255.

[8] Ogbonna, G., Caines, P.S., Catomeris, P., 1995. A competitive chemiluminescent immunoassay for the sensitive measurement of apolipoprotein B100. Clin. Biochem., 28:117-122.

[9] Schack, J.A., Waxler, S.H., 1949. An ultraviolet spectrophotometric method for the determination of theophylline and theobromine in blood and tissues. J. Pharmacol. Exp. Ther., 97(3):283-291.

[10] Shukla, G.S., Krag, D.N., 2005. Sensitive and rapid chemiluminescence ELISA for filamentous bacteriophages. J. Immunoassay Immunochem., 26(2):89-95.

[11] Solnica, B., 2004. Comparison of serum digoxin concentration monitoring by fluorescence polarization immunoassay on the TDxFLx and dry chemistry enzyme immunoassay on the Vitros 950. Clin. Chem. Lab. Med., 42:958-964.

[12] Terrier, N., Bonardet, A., Descomps, B., Cristol, J.P., Dupuy, A.M., 2004. Determination of beta2-microglobulin in biological samples using an immunoenzymometric assay (chemiluminescence detection) or an immunoturbidimetric assay: comparison with a radioimmunoassay. Clin. Lab., 50(11-12):675-683.

[13] Vergin, H., Mahr, G., Winterhalter, B., Wigand, R., 2003. Relative bioavailability and bioequivalence study of theophylline sustained release formulations. Arzneimittelforschung, 53(9):635-639.

[14] Yu, J.Y., Lin, Y., 2003. Clinical value comparison between chemiluminescent immunoassay and immunoradiometric assay in detecting TSH in serum. Hunan Yi Ke Da Xue Xue Bao, 28:275-277 (in Chinese).

[15] Zamora, B.M., Hartung, M., Hildebrandt, G., Kasbohrer, A., 1999. Detection of antibodies to S. enteritidis in broilers by means of indirect ELISA and chemiluminescent immunoassay (CLIA). Zentralbl Veterinarmed B, 46(1):9-23.