Journal of Zhejiang University SCIENCE B
(ISSN 1673-1581, Monthly)

2005   Vol. 6   No. 12   p. 1170-1175

            [ Home Page ] | [ PDF Full Text ]   On-line Access Date:   Nov. 22, 2005

Evaluation of ST13 gene expression in colorectal cancer patients

Dong Qing-hua, Zheng Shu†‡, Hu Yue, Chen Gong-xing, Ding Jia-yi

(Cancer Institute, Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310009, China)
 ‡ Corresponding Author
†E-mail: zhengshu@zju.edu.cn
Received July 28, 2005 revision accepted Oct. 4, 2005

Abstract: We identified a novel gene ST13 from a subtractive cDNA library of normal intestinal mucosa in 1993, more studies showed that ST13 was a co-chaperone of Hsp70s. Recently we detected the ST13 gene expression in tumor tissue and adjacent normal tissue of the same colorectal cancer patient and investigated if the ST13 gene expression might have any prognostic value. Analysis was performed at molecular level by reverse transcription-PCR using real-time detection method. We measured two genes simultaneously, ST13 as the target gene and glyceraldehydes-3-phosphate dehydrogenase as a reference gene, in primary colorectal tumor specimens and tumor-adjacent normal mucosa specimens from 50 colorectal cancer patients. The expression levels of the ST13 gene were significantly decreased in primary tumors compared with adjacent mucosa (P<0.05). But there were no significant differences in the expression of ST13 as compared with different Dukes’ stage, tumor differentiation grade, invasion depth, lymph node metastasis and disease-specific survival.

Key words: ST13, Colorectal cancer, Real-time PCR
doi:10.1631/jzus.2005.B1170             CLC number: R735

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