Journal of Zhejiang University SCIENCE A
(Monthly)

2006   Vol. 7   Suppl. II   p. 371-375

  ISSN 1009-3095(Print), 1862-1775(Online)
            [ Home Page ] | [ PDF Full Text ]   On-line Access Date:   Jul. 12, 2006

Effects of surfactant on biodesulfurization by Corynebacterium sp. ZD-1 in the presence of organic phase

Wang Miao-Dong†1,2, Li Wei2, Shi Yao2, Wang Da-Hui2, Feng Hai†‡1

(1College of Pharmacy, Zhejiang University of Technology, Hangzhou 310014, China)
(2Department of Environmental Engineering, Zhejiang University, Hangzhou 310027, China)
Corresponding Author
E-mail: wangmd9999@163.com; fenghai289289@163.com
Received Mar. 10, 2006 revision accepted June 5, 2006

Abstract: The effects of surfactants on dibenzothiophene (DBT) degradation by Corynebacterium sp. ZD-1 were investigated in hydrocarbon aqueous biphasic (O/W) systems in shake flask. Among Brij-35, Tween-80, Triton-100X and β-cyclodextrin, Tween-80 was a suitable surfactant to improve the desulfurization rate of dibenzothiophene. The amount of 2-hydroxybiphenyl (2-HBP) formed with Tween-80 present was about 50% more than that formed without surfactant. The results demonstrated that Tween-80 could improve the mass transfer of DBT between organic and aqueous phases, and could be used in dibenzothiophene biodesulfurization systems.

Key words: Biodesulfurization, Tween-80, Dibenzothiophene, Corynebacterium sp.
doi:10.1631/jzus.2006.AS0371             CLC number: TQ02

References:

[1] Gallardo, M.E., Ferrandez, A., de Lorenzo, V., Garcia, J.L., Diaz, E., 1997. Designing recombinant Pseudomonas strains to enhance biodesulfurization. J. Bacteriol., 179:7156-7160.

[2] Jiang, C.Y., Li, L., Yang, Y.T., Xing, J.M., Liu, H.Z., Chen, J.Y., 2002. Effect of surfactants on microbial removal of organic sulfur in diesel oil. The Chinese Journal of Process Engineering, 2(2):122-126 (in Chinese).

[3] Li, W., Zhang, Y., Wang, M.D., Shi, Y., 2005. Biodesulfurization of dibenzothiophene and other organic sulfur compounds by a newly isolated Microbacterium strain ZD-M2. FEMS Microbiol. Lett., 247(1):45-50.

[4] Luo, M.F., Xing, J.M., Gou, Z.X., Li, S., Liu, H.Z., Chen, J.Y., 2003. Desulfurization of dibenzothiophene by lyophilized cells of Pseudomonas delafieldii R-8 in the presence of dodecane. Biochemical Engineering Journal, 13:1-6.

[5] Ma, T., Wang, R.J., Liu, J., Liang, F.L., Liu, R.L., 2004. Experimental study on diesel circulating biodesulfurization. Petroleum Refinery Engineering, 34(1):52-54.

[6] Marzona, M., Pessione, E., Martino, S.D., Giunta, C., 1997. Benzothiophene and dibenzothiophene as the sole sulfur source in Acinetobacter: growth kinetics and oxidation products. Fuel Processing Technology, 52:199-205.

[7] Monticello, D.J., 1998. Riding the fossil fuel biodesulfurization wave. CHEMTECH, 28:38-45.

[8] Monticello, D.J., 2000. Biodesulfurization and the upgrading of petroleum distillates. Curr. Opin. Biotechnol., 11:540-546.

[9] Noda, K., Watanabe, K., Maruhashi, K., 2003. Isolation of a recombinant desulfurizing 4,6-diproply dibenzothiophene in n-tetradecane. J. Biosci. Bioeng., 95(4):354-360.

[10] Ohshiro, T., Izumi, Y., 1999. Microbial desufurization of organic sulfur compounds in petroleum. Biotechnol. Biochem., 63:1-9.

[11] Oldfield, C., Pogrebinsky, O., Simmonds, J., Oison, E.S., Kulpa, C.F., 1997. Elucidation of the metabolic pathway for dibenzothiophene desulfurization by Rhodococcus sp. Strain IGTS8 (ATCC 53968). Microbiology, 143:2961-2973.

[12] Samir, A.A., Claire, L., Michel, W., Frédéric, M., 2003. Multi-criteria comparison of resting cell activities of bacterial strains selected for biodesulfurization of petroleum compounds. Enzyme and Microbial Technology, 32:446-454.

[13] Setti, L., Bonoli, S., Badiali, E., Giuliani, S., 2003. Inverse phase transfer biocatalysis for a biodesulfurization process of middle distillates. BECTH. MOCK. УH-TA. CEP. 2.ХИМИЯ, 44(1):80-83.

[14] Sylvie, L.B., Rodolfo, Q., 2003. Biotechnological processes for the refining of petroleum. Fuel Processing Technology, 81:155-169.

[15] Wang, M.D., Li, W., Shi, Y., Wang, D.H., 2004a. Optimization of the growth conditions of Corynebacterium sp. ZD-1, a sulfur compounds-degradation bacterium. Journal of Zhejiang University (Engineering Science), 38(8):1020-1024 (in Chinese).

[16] Wang, M.D., Li, W., Wang, D.H., Shi, Y., 2004b. Desulfurization of dibenzothiophene by a newly isolated Corynebacterium sp. ZD-1 in aqueous phase. Journal of Environmental Science, 16(6):1011-1015.