Journal of Zhejiang University SCIENCE A
ISSN 1673-565X(Print), 1862-1775(Online), Monthly

2007   Vol. 8   No. 9   p. 1521~1526

Lovastatin production by Aspergillus terreus in solid-state fermentation

WEI Pei-lian^†1,2, XU Zhi-nan¹, CEN Pei-lin^†‡1

(¹Institute of Biochemical Engineering, Department of Chemical Engineering & Bioengineering, Zhejiang University, Hangzhou 310027, China)
(²Department of Biochemical Engineering, Zhejiang University of Science and Technology, Hangzhou 310023, China)
^‡ Corresponding Author
^†E-mail: weipeilian@126.com; cenpl@zju.edu.cn
Received Mar. 8, 2006 revision accepted Aug. 23, 2006

Abstract: Lovastatin production by Aspergillus terreus ATCC 20542 in solid-state fermentation (SSF) was studied. Various substrates were used to evaluate the ability of A. terreus to produce lovastatin. The results showed that either rice or wheat bran was suitable substrate for lovastatin production in SSF. The maximum yield of lovastatin (2.9 mg/g dry substrate) using rice as substrate was achieved after incubating for 11 d at the following optimized process parameters: 50%~60% initial moisture content, pH 5.5, incubation temperature 28 °C.

Key words: Solid-state fermentation (SSF), Rice, Lovastatin, Aspergillus terreus
doi:10.1631/jzus.2007.A1521             CLC number: Q93; R93

References:

[1] Abdel-Fattah, Y.R., Olama, Z.A., 2002. L-asparaginase production by Pseudomonas aeruginosa in solid-state culture: evaluation and optimization of culture conditions using factorial designs. Process Biochem., 38(1):115-122.

[2] Adinarayana, K., Prabhakar, T., Srinivasulu, V., Anitha, R.M., Jhansi, L.P., Ellaiah, P., 2003. Optimization of process parameters for cephalosporin C production under solid state fermentation from Acremonium chrysogenum. Process Biochem., 39(2):171-177.

[3] Arima, K., Uozumi, T., 1967. A new method for estimation of the mycelial weight in Koji. Agric. Biol. Chem., 31(1):119-123.

[4] Balakrishnan, K., Pandey, A., 1996. Production of biologically active secondary metabolites in solid state fermentation. J. Sci. Ind. Res., 55(2):365-372.

[5] Casas López, J.L., Sánchez Pérez, J.A., Fernández Sevilla, J.M., Acién Fernández, F.G., Molina Grima, E., Chisti, Y., 2003. Production of lovastatin by Aspergillus terreus: effects of the C:N ratio and the principal nutrients on growth and metabolite production. Enzyme Microbiol. Technol., 33(2-3):270-277.

[6] Cen, P.L., Xia, L.M., 1999. Cellulase production by solid state fermentation. Adv. Biochem. Eng. Biotechnol., 65(1): 69-92.

[7] Chang, Y.N., Huang, J.C., Lee, C.C., Shih, I.L., Tzeng, Y.M., 2002. Use of response surface methodology to optimize culture medium for production of lovastatin by Monascus ruber. Enzyme Microbiol. Technol., 30(7):889-894.

[8] Endo, A., 1979. Monacolin, K, a new hypocholesterolemic agent produced by a Monascus species. J. Antibiot., 32(8):852-854.

[9] Frishman, W.H., Zimetbaum, P., Nadelmann, J., 1989. Lovastatin and other HMG-CoA reductase inhibitors. J. Clin. Pharmacol., 29(3):975-982.

[10] Gombert, A.K., Pinto, A.L., Castilho, L.R., Freire, D.M.G., 1999. Lipase production by Penicillium restrictum in solid state fermentation using babassu oil cake as substrate. Process Biochem., 35(1-2):85-90.

[11] Hölker, U., Lenz, J., 2005. Solid-state fermentation—are there any biotechnological advantages? Curr. Opin. Microbiol., 8(3):301-306.

[12] Hölker, U., Höfer, M., Lenz, J., 2004. Biotechnological advantages of laboratory-scale solid-state fermentation with fungi. Appl. Microbiol. Biotechnol., 64(2):175-186.

[13] Koliander, B., Hampel, W., Roehr, M., 1984. Indirect estimation of biomass by rapid ribonucleic acid determination. Appl. Microbiol. Biotechnol., 19(2):272-276 .

[14] Liu, G., 1997. Kinetics in Antibiotics Fermentation. Ph.D Thesis, Zhejiang University, China (in Chinese).

[15] Liu, G., Xu, Z.N., Cen, P.L., 2000. A morphologically structured model for mycelial growth and secondary metabolite formation. Chinese J. Chem. Eng., 8(1):46-51.

[16] Ohno, A., Ano, T., Shoda, M., 1993. Production of the antifungal peptide antibiotic, iturin by Bacillus subtilis NB22 in solid state fermentation. J. Ferm. Bioeng., 75(1):23-27.

[17] Ramana Murthy, M.V., Mohan, E.V.S., Sadhukhan, A.K., 1999. Cyclosporin-A production by Tolypocladium inflatum using solid state fermentation. Process Biochem., 34(3):269-280.

[18] Shojaosadati, S.A., Babaeipour, V., 2002. Citric acid production from apple pomace in multi-layer packed bed solid-state bioreactor. Process Biochem., 37(8):909-914.

[19] Sitaram Kumar, M., Jana, S.K., Senthil, V., Shashanka, V., Vijay Kumar, S., Sadhukhan, A.K., 2000. Repeated fed-batch process for improving lovastatin production. Process Biochem., 36(4):363-368.

[20] Valera, H.R., Gomes, J., Lakshmi, S., Gururaja, R., Suryanarayan, S., Kumar, D., 2005. Lovastatin production by solid state fermentation using Aspergillus flavipes. Enzym. Microb. Tech., 37(5):521-526.

[21] Xia, L.M., Cen, P.L., 1999. Cellulase production by solid state fermentation on lignocellulosic waste from the xylose industry. Process Biochem., 34(9):909-912.

[22] Xu, B.J., Wang, Q.J., Jia, X.Q., Sung, C.K., 2005. Enhanced lovastatin production by solid state fermentation of Monascus ruber. Biotechnol. Bioproc. Eng., 10(1):78-84.