Journal of Zhejiang University SCIENCE B
ISSN 1673-1581(Print), 1862-1783(Online), Monthly

2008   Vol. 9   No. 9   p. 701~706

Arylamine N-acetyltransferases: a new inhibitor of apoptosis in HepG2 cells

Yu-bin JI^1,2, Shi-yong GAO^†‡1,2

(¹Postdoctoral Research Station of the Institute of Materia Medica, Center for Life Sciences and Environmental Sciences, Harbin University of Commerce, Harbin 150076, China)
(²MOE Engineering Research Center of Natural Anticancer Drugs, Harbin University of Commerce, Harbin 150076, China)
^‡ Corresponding Author
^†E-mail: sygao2002@163.com
Received Mar. 20, 2008; revision accepted July 29, 2008

Abstract: Objective: To explore how arylamine N-acetyltransferases (NATs) is related to cell apoptosis. Methods: NAT activity in apoptotic HepG2 cells was measured using high performance liquid chromatography (HPLC); the apoptosis rate of HepG2 cells acted upon by an NAT inhibitor was measured using flow cytometry. Results: NAT activity was lowered in apoptotic HepG2 cells; apoptosis rate induced by camptothecin (CAM) increased after inhibition of NAT activity in HepG2 cells. Conclusion: NAT can inhibit apoptosis in HepG2 cells.

Key words: Arylamine N-acetyltransferases (NATs), Apoptosis, HepG2, Camptothecin (CAM)
doi:10.1631/jzus.B0820090             CLC number: Q81

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