Journal of Zhejiang University SCIENCE B
ISSN 1673-1581(Print), 1862-1783(Online), Monthly

2008   Vol. 9   No. 9   p. 728~733

Simultaneous detection of seven phenolic acids in Danshen injection using HPLC with ultraviolet detector

Jin-zhong XU, Jie SHEN, Yi-yu CHENG, Hai-bin QU^†‡

(Department of Chinese Medicine Science and Engineering, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China)
^‡ Corresponding Author
^†E-mail: quhb@zju.edu.cn
Received Mar. 20, 2008; revision accepted Apr. 24, 2008

Abstract: A high-performance liquid chromatographic (HPLC) method with ultraviolet (UV) detector had been developed for simultaneous quantification of danshensu, protocatechuic aldehyde, caffeic acid, salvianolic acid D, rosmarinic acid, salvianolic acid B and salvianolic acid A in Danshen injection. According to the UV spectra of these components, three detection wavelengths have been selected as follows: 280 nm for danshensu and protocatechuic aldehyde, 326 nm for caffeic acid, salvianolic acid D and rosmarinic acid, 286 nm for salvianolic acid B and salvianolic acid A. The limit of detection (LOD) was improved to be in the range of 0.008~0.160 μg/ml. Moreover, excellent linear behavior over the investigated concentration range was observed, with R>0.999 for all the analytes.

Key words: High-performance liquid chromatography (HPLC), Danshen injection (DSI), Phenolic acids, Multi-wavelengths, Quantification
doi:10.1631/jzus.B0820095             CLC number: R91

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