Journal of Zhejiang University SCIENCE A
ISSN 1673-565X(Print), 1862-1775(Online), Monthly

2009   Vol. 10   No. 1   p. 133~141

Applicability of anoxic-oxic process in treating petrochemical wastewater

Li-jun ZHAO^†1,2, Fang MA², Jing-bo GUO²

(¹School of Chemical Engineering, China University of Petroleum, Beijing 102249, China)
(²School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, China)
^†E-mail: wwep001@yahoo.com.cn
Received Jan. 2, 2008; revision accepted June 26, 2008

Abstract: To explore the applicability of anoxic-oxic (A/O) activated sludge process for petrochemical wastewater treatment, the relationship between bacterial community structure and pollutants loading/removal efficiencies was investigated by gas chromatograph-mass spectrometry (GC-MS), polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) and other conventional techniques. It showed that when the concentrations of the influent chemical oxygen demand (COD) and ammonia nitrogen (NH₄⁺-N) were 420~560 mg/L and 64~100 mg/L, respectively, the corresponding average effluent concentrations were 160 mg/L and 55 mg/L, which were 1.6 and 2.2 times higher than those of the national standards in China, respectively, demonstrating the inefficient performances of A/O process. Analysis of GC-MS indicated that refractory pollutants were mainly removed by sludge adsorption, but not by biodegradation. PCR-DGGE profile analysis suggested that the biological system was species-rich, but there was apparent succession of the bacterial community structure in different locations of the A/O system. Variations of bacterial community structure and pollutant loadings had obvious influences on pollutants removal efficiencies. Thus, A/O process was inapplicable for the treatment of complicated petrochemical wastewater, and strategies such as the reinforcement of pre-treatment and two-stage A/O process were suggested.

Key words: Petrochemical wastewater, Polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE), Anoxic-oxic (A/O) process, Applicability,
doi:10.1631/jzus.A0820006             CLC number: X703

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