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Journal of Zhejiang University SCIENCE  2003 Vol.4 No.3 P.346~351

doi: 10.1631/jzus.2003.0346


Agrobacterium-mediated transformation of herbicide resistance in creeping bentgrass and colonial bentgrass


Author(s):  CHAI Ming-liang, WANG Bing-liang, KIM Jae-yeoul, LEE Jong-min, KIM Doo-hwan

Affiliation(s):  Department of Horticulture, Zhejiang University, Hangzhou 310029, China; more

Corresponding email(s):  mlchai@zju.edu.cn

Key Words:  Agrostis palustris, Agrostis tenuis, Agrobacterium, Herbicide resistance, sGFP(synthetic green fluorescent protein)


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CHAI Ming-liang, WANG Bing-liang, KIM Jae-yeoul, LEE Jong-min, KIM Doo-hwan. Agrobacterium-mediated transformation of herbicide resistance in creeping bentgrass and colonial bentgrass[J]. Journal of Zhejiang University Science , 2003, 4(18): 346~351.

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author="CHAI Ming-liang, WANG Bing-liang, KIM Jae-yeoul, LEE Jong-min, KIM Doo-hwan",
journal="Journal of Zhejiang University Science ",
volume="4",
number="3",
pages="346~351",
year="2003",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.2003.0346"
}

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%A CHAI Ming-liang
%A WANG Bing-liang
%A KIM Jae-yeoul
%A LEE Jong-min
%A KIM Doo-hwan
%J Journal of Zhejiang University SCIENCE
%V 4
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%P 346~351
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%I Zhejiang University Press & Springer

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T1 - Agrobacterium-mediated transformation of herbicide resistance in creeping bentgrass and colonial bentgrass
A1 - CHAI Ming-liang
A1 - WANG Bing-liang
A1 - KIM Jae-yeoul
A1 - LEE Jong-min
A1 - KIM Doo-hwan
J0 - Journal of Zhejiang University Science
VL - 4
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SP - 346
EP - 351
%@ 1869-1951
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PB - Zhejiang University Press & Springer
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Abstract: 
Embryogenic calli were induced from the seeds of creeping bentgrass (agrostis palustris Huds.) cv. Regent and colonial bentgrass (agrostis tenuis Sibth. Fl. Oxen.) cv. Tiger. The embryogenic calli were precultured on fresh medium for 4-7 days and then co-cultivated with agrobacterium tumefaciens, LBA4404, which contains plasmid vector-pSBGM harboring bar coding region, synthetic green fluorescent protein (sGFP) coding region and matrix attachment region (MAR). After 3 days of co-cultivation, the calli were washed thoroughly and transferred to MS medium containing 2 mg/L of 2, 4-D, 12-15 mg/L phosphinothricin (PPT) and 250 mg/L of cefotaxime. After 2-3 months of selection, the actively growing calli of 'Regent' and 'Tiger' were transferred to MS medium with 12-15 mg/L PPT and 250 mg/L cefotaxime for regeneration. The putative transformants were maintained on MS medium with 3 mg/L PPT for long period but control died within 1 month. After establishing in greenhouse, the transformants also showed strong resistance to 0.4% of herbicide Basta but control plants died within 2 weeks. Under confocal microscope, both young leaves and roots showed significant GFP expression. PCR analysis revealed the presence of a DNA fragment of GFP gene at the expected size (380 bp) in the transformants and its absence in a randomly selected control plant.

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Reference

[1]Asano, Y., Ito, Y., Fukami, M., Morifuji, M. and Fujiie, A., 1997. Production of herbicide resistant, transgenic creeping bentgrass plants. International Turfgrass Research Journal, 8:261-267.

[2]Birch, R. G. and Frank, T., 1991. Development and optimization of microprojetile systems for plant genetic transformation. Aus. J. Plant Physiol., 18:453-469.

[3]Blanch, F. C., Krans, J. V. and Coat, G. E., 1986. Improvement in callus growth and plantlets formation in creeping bentgrass. Crop Sci., 26:1245-1248.

[4]Chai, M.L. and Kim, D. H., 1998. Effect of amino acids on callus induction from bentgrass. Korean Journal of Turfgrass Science, 12(4):189-194.

[5]Chai, M.L., Senthil, K., Mo, S. Y., Chung, Y. S., Cho, S. H., Shin, J. S., Park, M. H. and Kim, D. H., 2000. Embryogenic callus induction and Agrobacterium-mediated transformation in bentgrass (Agrostis spp.). J. Kor. Soc. Hort. Sci., 41:450-454.

[6]Chilton, M. D., Currier, T. C., Farand, S. K., Bendich, A. J., Gordon, M. P. and Nester, E. W., 1974. Agrobacterium tumefaciens DNA and PS8 bacteriophage DNA not detected in crown gall tumors. Proc. Natl. Acad. Sci., 71:3672-3676.

[7]Deblock, M., 1993. The cell biology of plant transformation: Culture state, problems, prospects and the implications for the plant breeding. Euphytica, 71:1-14.

[8]D'Halluin, K., De Block, M., Denecke, J., Janssens, J., Leemans, J., Reynaerts, A., and Botterman, J., 1992. The bar gene as selectable and screenable marker in plant engineering. In: Methods in Enzymology. Wu R (ed), Academic Press, Inc, CA,p.415-416.

[9]Hartman, C. L., Lee, L., Day, P. R. and Tumer, N. E., 1994. Herbicide resistant turfgrass (Agrostis palustris Hunds.) by biolistic transformation. Bio/Technology, 12:919-923.

[10]Hashizume, F., Tsuchiya, T., Ugaki, M., Niwa, Y., Tachibana, N. and Kowyama, Y., 1999. Efficient Agrobacterium-mediated transformation and the usefulness of a synthetic GFP reporter gene in leading varieties of Japanese rice. Plant Biotechnology, 15:398-401.

[11]Hiei, Y., Ohta, S., Komari, T. and Kumashiro, T., 1994. Efficient transformation of rice (Oryza sativa L.) mediated by Agrobacterium and sequence analysis of the boundaries of the T-DNA. The Plant Journal, 6:271-282.

[12]Horsch, R. B., Fry, J., Hoffmann, N., Wallroth, M., Eichholtz, S. G. and Fraley, R. T., 1985. A simple and general methods for transferring genes into plants. Science, 227:1229-1231.

[13]Krans, J. V., Henning, V. T. and Torres, K. C., 1982. Callus induction, maintenance and plantlet regeneration in creeping bentgrsass. Crop Sci., 22:1193-1197.

[14]Lee, L., Laramore, C. L., Day, P. R. and Tumer, N. E., 1996. Transformation and regeneration of creeping bentgrass (Agrostis palustris Hunds.) protoplasts. Crop Sci., 36:401-406.

[15]Liu, C. A., Zhong, H., Vargas, J., Penner, D. and Sticklen, M., 1998. Prevention of fungal diseases in transgenic, bialophos and glufosinate-resistant creeping bentgrass (Agrostis palustris L.). Weed Science, 46:139-146.

[16]Marray, M. and Thompson, W. F., 1980. Rapid isolation of high molecular weight plant DNA. Nucleic Acids Res., 8:4321-4325.

[17]Murashige, T. and Skoog, F., 1962. A reversed medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant., 15:473-497.

[18]Niedz, R. P., Sussman, M. R. and Satterlee, J. S., 1995. Green fluorescent protein: An in vitro reporter of plant gene expression. Plant Cell Rep., 14:403-406.

[19]Smith, R. H. and Hood, E. E., 1995. Agrobacterium tumefaciens transformation of monocots. Crop Sci., 35:301-309.

[20]Terakawa, T., Sato, T. and Koike, M., 1992. Plant regeneration from protoplasts isolated from embryogenic suspension cultures of creeping bentgrass (Agrostis palustris Huds.). Plant Cell Rep., 11:457-461.

[21]Warkentin, D., Chai, B., Hajela, R. K., Hajela, P. K., Zhong, H. and Sticklen, M. B., 1997. Development of transgenic creeping bentgrass (Agrostis palustris Huds.) for fungal diseases resistance. In: Sticklen M B and Kenna M P (Eds.) Turfgrass Biology-Cell and molecular genetic approaches to turfgrass improvement. Ann Arbor Press, MI, p.153-161.

[22]Yu, T. T., Skinner, D. Z., Liang, G. H., Trick, H. N., Huang, B. and Muthukrishnan, S., 2000. Agrobacterium-mediated transformation of creeping bentgrass using GFP as a reporter gene. Hereditas, 133:229-233.

[23]Zhong, H., Srinivasan, C. and Sticklen, M. B., 1991. Plant regeneration via somatic enbryogenesis in creeping bentgrass (Agrostis palustris Hunds.). Plant Cell Rep., 10:453-456.

[24]Zhong, H., Bolyard, M. G., Srinivasan, C. and Sticklen, M. B., 1993. Transgenic plants of turfgrass (Agrostis palustris Huds.) from microprojectile bombardment of embryogenic callus. Plant Cell Rep., 13:1-6.

[25]Zhong, H., Liu, C. A., Vargas, Jr. J. M., Penner, D., and Sticklen, M. B., 1997. Simultaneous control of weeds, dollar spot, and brown patch diseases in transgenic creeping bentgrass. In: Sticklen M B and Kenna M P (ed.). Turfgrass biotechnology-Cell and molecular genetic approaches to turfgrass improvement. Ann Arbor Press, MI, p.203-210.

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