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CLC number: R735

On-line Access: 2013-03-06

Received: 2012-04-01

Revision Accepted: 2012-09-03

Crosschecked: 2013-02-01

Cited: 1

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Citations:  Bibtex RefMan EndNOte GB/T7714

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Journal of Zhejiang University SCIENCE B 2013 Vol.14 No.3 P.216-223

doi: 10.1631/jzus.B1200102


Retracted Article: Characterization of cancer stem-like cells in the side population cells of human gastric cancer cell line MKN-45


Author(s):  Hai-hong Zhang, Ai-zhen Cai, Xue-ming Wei, Li Ding, Feng-zhi Li, Ai-ming Zheng, Da-jiang Dai, Rong-rong Huang, Hou-jun Cao, Hai-yang Zhou, Jian-mei Wang, Xue-jing Wang, Wei Shi, Heng Zhu, Xiao-ying Yuan, Lin Chen

Affiliation(s):  1.Department of General Surgery, General Hospital of the Air Force, PLA, Beijing 100142, China; Department of General Surgery, Chinese PLA General Hospital, Beijing 100853, China; Department of Cell Biology, Institute of Basic Medical Sciences, Academy of Military Medical Sciences, Beijing 100850, China

Corresponding email(s):  862831598@qq.com , chenlinbj@vip.sina.com

Key Words:  ATP-binding cassette transporters, Side population cells, Stem cells, Benzimidazole (Hoechst 33342), 10.1631/jzus.B12r0102

"&ck%5B%5D=keyword&opd=AND"> Stomach neoplasmRetraction Note of this article can be found at doi:10.1631/jzus.B12r0102


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Hai-hong Zhang, Ai-zhen Cai, Xue-ming Wei, Li Ding, Feng-zhi Li, Ai-ming Zheng, Da-jiang Dai, Rong-rong Huang, Hou-jun Cao, Hai-yang Zhou, Jian-mei Wang, Xue-jing Wang, Wei Shi, Heng Zhu, Xiao-ying Yuan, Lin Chen. Retracted Article: Characterization of cancer stem-like cells in the side population cells of human gastric cancer cell line MKN-45[J]. Journal of Zhejiang University Science B, 2013, 6(14): 216-223.

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title="Retracted Article: Characterization of cancer stem-like cells in the side population cells of human gastric cancer cell line MKN-45",
author="Hai-hong Zhang, Ai-zhen Cai, Xue-ming Wei, Li Ding, Feng-zhi Li, Ai-ming Zheng, Da-jiang Dai, Rong-rong Huang, Hou-jun Cao, Hai-yang Zhou, Jian-mei Wang, Xue-jing Wang, Wei Shi, Heng Zhu, Xiao-ying Yuan, Lin Chen",
journal="Journal of Zhejiang University Science B",
volume="14",
number="6",
pages="216-223",
year="2013",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1200102"
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%0 Journal Article
%T Retracted Article: Characterization of cancer stem-like cells in the side population cells of human gastric cancer cell line MKN-45
%A Hai-hong Zhang
%A Ai-zhen Cai
%A Xue-ming Wei
%A Li Ding
%A Feng-zhi Li
%A Ai-ming Zheng
%A Da-jiang Dai
%A Rong-rong Huang
%A Hou-jun Cao
%A Hai-yang Zhou
%A Jian-mei Wang
%A Xue-jing Wang
%A Wei Shi
%A Heng Zhu
%A Xiao-ying Yuan
%A Lin Chen
%J Journal of Zhejiang University SCIENCE B
%V 14
%N 6
%P 216-223
%@ 1673-565X
%D 2013
%I Zhejiang University Press & Springer

TY - JOUR
T1 - Retracted Article: Characterization of cancer stem-like cells in the side population cells of human gastric cancer cell line MKN-45
A1 - Hai-hong Zhang
A1 - Ai-zhen Cai
A1 - Xue-ming Wei
A1 - Li Ding
A1 - Feng-zhi Li
A1 - Ai-ming Zheng
A1 - Da-jiang Dai
A1 - Rong-rong Huang
A1 - Hou-jun Cao
A1 - Hai-yang Zhou
A1 - Jian-mei Wang
A1 - Xue-jing Wang
A1 - Wei Shi
A1 - Heng Zhu
A1 - Xiao-ying Yuan
A1 - Lin Chen
J0 - Journal of Zhejiang University Science B
VL - 14
IS - 6
SP - 0
EP - 0
SN - 1673-565X
Y1 - 2013
PB - Zhejiang University Press & Springer
ER -


Abstract: 
Objective: Side population (SP) cells may play a crucial role in tumorigenesis and the recurrence of cancer. Many kinds of cell lines and tissues have demonstrated the presence of SP cells, including several gastric cancer cell lines. This study is aimed to identify the cancer stem-like cells in the SP of gastric cancer cell line MKN-45. Methods: We used fluorescence activated cell sorting (FACS) to sort SP cells in the human gastric carcinoma cell line MKN-45 (cells labeled with Hoechst 33342) and then characterized the cancer stem-like properties of SP cells. Results: This study found that the SP cells had higher clone formation efficiency than major population (MP) cells. Five stemness-related gene expression profiles, including OCT-4, SOX-2, NANOG, CD44, and adenosine triphosphate (ATP)-binding cassette transporters gene ABCG2, were tested in SP and MP cells using quantitative real-time reverse transcription polymerase chain reaction (RT-PCR). Western blot was used to show the difference of protein expression between SP and MP cells. Both results show that there was significantly higher protein expression in SP cells than in MP cells. When inoculated into non-obese diabetic/severe combined immunodeficiency (NOD/SCID) mice, SP cells show higher tumorigenesis tendency than MP cells. Conclusions: These results indicate that SP cells possess cancer stem cell properties and prove that SP cells from MKN-45 are gastric cancer stem-like cells.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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