Imaging and mathematical modeling of the bladderwort Utricularia gibba show how carnivorous plant traps shape themselves in 3D by growth oriented by a tissue polarity field. Credit: Karen Lee, Yohei Koide, John Fozard and Claire Bushell.
Leaves display a remarkable range of forms from flat sheets with simple outlines to the cup-shaped traps found in carnivorous plants.
A general question in developmental and evolutionary biology is how tissues shape themselves to create the diversity of forms we find in nature such as leaves, flowers, hearts, and wings.
Study of leaves has led to progress in understanding the mechanisms that produce the simpler, flatter forms. But it’s been unclear what lies behind the more complex curved leaf forms of carnivorous plants.
Previous studies using the model species Arabidopsis thaliana which has flat leaves revealed the existence of a polarity field running from the base of the leaf to the tip, a kind of inbuilt cellular compass which orients growth.
To test if an equivalent polarity field might guide growth of highly curved tissues, researchers analyzed the cup-shaped leaf traps of the aquatic carnivorous plant Utricularia gibba, commonly known as the humped bladderwort.
The team of Professor Enrico Coen used a combination of 3D imaging, cell and clonal analysis, and computational modeling to understand how carnivorous plant traps are shaped.
These approaches showed how Utricularia gibba traps grow from a near-spherical ball of cells into a mature trap capable of capturing prey.
By measuring 3D snapshots of traps at various developmental stages and exploring computational growth models they showed how differential rates and orientations of growth are involved.
The team used fluorescent proteins to monitor cellular growth directions and 3D imaging at different developmental stages to study the changing shape of the trap.
The computational modeling used to account for oriented growth invokes a polarity field comparable to that proposed for Arabidopsis leaf development, except that here it propagates within a curved sheet.
Analysis of the orientation of quadrifid glands, which in Utricularia gibba are used for nutrient absorption, confirmed the existence of the hypothesized polarity field.
The study which appears in the Journal PLOS Biology concludes that simple modulation of mechanisms underlying flat leaf development can also account for the shaping of more complex 3D shapes.
One of the lead authors Karen Lee said, “A polarity field orienting growth of tissue sheets may provide a unified explanation behind the development of the diverse range of leaves we find in nature.”
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The study, done in collaboration with the group of Minlong Cui at Zhejiang University in China, appears in PLOS Biology: Shaping of a three-dimensional carnivorous trap through modulation of a planar growth mechanism.
The work was funded by the European Research Council (Carnomorph grant number 323028) and the Biological Sciences Research Council (grant nos. BB/M023117/1, BB/L008920/1, BB/P020747/1 and BBS/E/J/000PR978
Reference: “Shaping of a three-dimensional carnivorous trap through modulation of a planar growth mechanism” by Karen J. I. Lee, Claire Bushell, Yohei Koide, John A. Fozard, Chunlan Piao, Man Yu, Jacob Newman, Christopher Whitewoods, Jerome Avondo, Richard Kennaway, Athanasius F. M. Marée, Minlong Cui and Enrico Coen, 10 October 2019, PLOS Biology.
DOI: 10.1371/journal.pbio.3000427
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